HALO® BioClass UHPLC & HPLC columns for the separation of peptides and polypeptides

temperature-stable up to 90°C

broad pH range (pH 1 - 8)

high peak resolution, low back pressure

rugged and reliable, may be used on UHPLC as well as standard HPLC instruments

As research in proteomics has intensified, more demands have been placed on
- columns with high resolution and peak capacity
- columns to better analyse complex samples
- faster columns that speed up analysis time and improve laboratory productivity

The HALO® BioClass Fused Core columns were specifically designed for the fast separation of peptides and proteins. Specifically selected pore sizes, optimized porous shell thickness and particle size, and the use of extraordinary stable bonding chemistry provides optimum separation of peptides up to 10 kDa (HALO® Peptide) and proteins and polypeptides in the range of 2 kDa to 500 kDa (HALO® Protein). The low back-pressure of these columns permit them to be used with either UHPLC or conventional HPLC equipment.

 
     

High peak resolution

HALO®-5 Peptide ES-C18
HALO®-5 Peptide ES-C18 provides improved resolution compared to a typical totally-porous wide-pore column

 

Low back pressure

By virtue of their optimized particle sizes, HALO® BioClass columns generate significantly less back pressure compared to sub-2 μm UHPLC columns. The low back pressure allows the use of standard HPLC equipment.

     
Fast separation    

Fast separation of peptides and small proteins

  Fast separation of proteins
     
High temperature stability

The temperature stability of HALO® Peptide and HALO® Protein columns allows them to be used at elevated temperature to improve resolution or to provide better protein recovery and peak shape. Operating at elevated column temperature is especially valuable when analysing large hydrophobic proteins.

HALO® Peptide and HALO® Protein columns demonstrate outstanding temperature stability   Effect of temperature on protein recovery and peak shape
     
     

HALO®-5 Peptide ES-C18

Phase: Octadecyldiisobutylsilane, no endcapping

particle size: 4.6 µm
solid core: 3.5 µm silica
shell: 0.6 µm porous silica

pore size: 160 Å
application: peptides MW 5 -10 kDa

HALO® Peptide ES-C18

Phase: Octadecyldiisobutylsilane, no endcapping

particle size: 2.7 µm
solid core: 1.7 µm silica
shell: 0.5 µm porous silica

pore size: 160 Å
application: peptides MW 5 - 20 kDa

Peptide ES-C18 is a C18 phase bonded to a Fused-Core particle with a pore size (160Å) specifically selected for ultra-fast, high-resolution separation of peptides up to 20 kDa.

The mechanism of separation offered by the bonded phase is hydrophobic binding interaction, well suited for peptide separations. The pore size is selected to balance the importance of allowing peptides full access to the stationary phase pore volume while maintaining sufficient surface area for analyte-stationary phase-mobile phase interaction. Furthermore, the bonded phase is sterically-protected to facilitate reliable operations under the low pH and high temperature conditions that are often used for separating peptides by reversed phase.

 

HALO®-5 Peptide ES-CN

Phase: Cyanopropyldiisopropylsilane, fully endcapped

particle size: 4.6 µm
solid core: 3.5 µm silica
shell: 0.6 µm porous silica

pore size: 160 Å
application: peptides MW 3 kDa ≤ MW ≤ 20 kDa

HALO® Peptide ES-CN

Phase: Cyanopropyldiisopropylsilane, fully endcapped

particle size: 2.7 µm
solid core: 1.7 µm silica
shell: 0.5 µm porous silica

pore size: 160 Å
application: peptides 3 kDa ≤ MW ≤ 20 kDa

Alternative phase with reduced retention and alternative selectivity for HPLC and UHPLC separation of peptides and proteins from 3 kDa ≤ MW ≤ 20 kDa. Sterically protected ligands provide improved stability at low pH and at temperatures up to 90°C

             
Dimension HALO®-5
Peptide ES-C18
HALO®
Peptide ES-C18
  Dimension HALO®-5
Peptide ES-CN
HALO®
Peptide ES-CN
0.75 x 50 mm 8AM-91529-402 8AM-91229-402   0.075 x 50 mm 8AM-91529-404 8AM-91229-404
0.075 x 150 mm 8AM-91529-702 8AM-91229-702   0.075 x 150 mm 8AM-91529-704 8AM-91229-704
0.10 x 50 mm 8AM-91528-402 8AM-91228-402   0.10 x 50 mm 8AM-91528-404 8AM-91228-404
0.10 x 150 mm 8AM-91528-702 8AM-91228-702   0.10 x 150 mm 8AM-91528-704 8AM-91228-704
0.20 x 50 mm 8AM-91527-402 8AM-91227-402   0.20 x 50 mm 8AM-91527-404 8AM-91227-404
0.20 x 150 mm 8AM-91527-702 8AM-91227-702   0.20 x 150 mm 8AM-91527-704 8AM-91227-704
0.30 x 50 mm 8AM-91526-402 8AM-91226-402   0.30 x 50 mm 8AM-91526-404 8AM-91226-404
0.30 x 150 mm 8AM-91526-702 8AM-91226-702   0.30 x 150 mm 8AM-91526-704 8AM-91226-704
0.50 x 50 mm 8AM-91525-402 8AM-91225-402   0.50 x 50 mm 8AM-91525-404 8AM-91225-404
0.50 x 150 mm 8AM-91525-702 8AM-91225-702   0.50 x 150 mm 8AM-91525-704 8AM-91225-704
1.0 x 30 mm 8AM-95121-302 8AM-92121-302   1.0 x 30 mm 8AM-95121-304 8AM-92121-304
1.0 x 50 mm 8AM-95121-402 8AM-92121-402   1.0 x 50 mm 8AM-95121-404 8AM-92121-404
1.0 x 100 mm 8AM-95121-602 8AM-92121-602   1.0 x 100 mm 8AM-95121-604 8AM-92121-604
1.0 x 150 mm 8AM-95121-702 8AM-92121-702   1.0 x 150 mm 8AM-95121-704 8AM-92121-704
2.1 x 20 mm 8AM-95122-202 8AM-92122-202   2.1 x 20 mm 8AM-95122-204 8AM-92122-204
2.1 x 30 mm 8AM-95122-302 8AM-92122-302   2.1 x 30 mm 8AM-95122-304 8AM-92122-304
2.1 x 50 mm 8AM-95122-402 8AM-92122-402   2.1 x 50 mm 8AM-95122-404 8AM-92122-404
2.1 x 75 mm 8AM-95122-502 8AM-92122-502   2.1 x 75 mm 8AM-95122-504 8AM-92122-504
2.1 x 100 mm 8AM-95122-602 8AM-92122-602   2.1 x 100 mm 8AM-95122-604 8AM-92122-604
2.1 x 150 mm 8AM-95122-702 8AM-92122-702   2.1 x 150 mm 8AM-95122-704 8AM-92122-704
2.1 x 250 mm 8AM-95122-902 -   2.1 x 250 mm 8AM-95122-904 -
3.0 x 30 mm 8AM-95123-302 8AM-92123-302   3.0 x 30 mm 8AM-95123-304 8AM-92123-304
3.0 x 50 mm 8AM-95123-402 8AM-92123-402   3.0 x 50 mm 8AM-95123-404 8AM-92123-404
3.0 x 75 mm 8AM-95123-502 8AM-92123-502   3.0 x 75 mm 8AM-95123-504 8AM-92123-504
3.0 x 100 mm 8AM-95123-602 8AM-92123-602   3.0 x 100 mm 8AM-95123-604 8AM-92123-604
3.0 x 150 mm 8AM-95123-702 8AM-92123-702   3.0 x 150 mm 8AM-95123-704 8AM-92123-704
3.0 x 250 mm 8AM-95123-902 -   3.0 x 250 mm 8AM-95123-904 -
4.6 x 30 mm 8AM-95124-302 8AM-92124-302   4.6 x 30 mm 8AM-95124-304 8AM-92124-304
4.6 x 50 mm 8AM-95124-402 8AM-92124-402   4.6 x 50 mm 8AM-95124-404 8AM-92124-404
4.6 x 75 mm 8AM-95124-502 8AM-92124-502   4.6 x 75 mm 8AM-95124-504 8AM-92124-504
4.6 x 100 mm 8AM-95124-602 8AM-92124-602   4.6 x 100 mm 8AM-95124-604 8AM-92124-604
4.6 x 150 mm 8AM-95124-702 8AM-92124-702   4.6 x 150 mm 8AM-95124-704 8AM-92124-704
4.6 x 250 mm 8AM-95124-902 -   4.6 x 250 mm 8AM-95124-904 -
10 x 50 mm 8AM-95110-402 8AM-92110-402   10 x 50 mm 8AM-95110-404 8AM-92110-404
10 x 100 mm 8AM-95110-602 8AM-92110-602   10 x 100 mm 8AM-95110-604 8AM-92110-604
10 x 150 mm 8AM-95110-702 8AM-92110-702   10 x 150 mm 8AM-95110-704 8AM-92110-704
10 x 250 mm 8AM-95110-902 -   10 x 250 mm 8AM-95110-904 -
             

HALO® Peptide ES-C18 guard columns

Guard column holder: 8AM-94900-001

 

HALO® Peptide ES-CN guard columns

Guard column holder: 8AM-94900-001

2.1 x 5 mm 8AM-95122-102 8AM-92122-102   2.1 x 5 mm 8AM-95122-104 8AM-92122-104
3.0 x 5 mm 8AM-95123-102 8AM-92123-102   3.0 x 5 mm 8AM-95123-104 8AM-92123-104
4.6 x 5 mm 8AM-95124-102 8AM-92124-102   4.6 x 5 mm 8AM-95124-104 8AM-92124-104
             

HALO® Protein C4

Phase: Dimethylbutylsilane, fully endcapped

particle size: 3.4 µm
solid core: 3.0 µm silica
shell: 0.2 µm porous silica

pore size: 400 Å
application: proteins and polypeptides
range: MW 2 kDa – 500 kDa

Specifically developed for the fast separation of proteins and polypeptides up to Mw 500 kDa. High temperature stability.

HALO® Protein ES-C18

Phase: Octadecyldiisobutylsilane, no endcapping

particle size: 3.4 µm
solid core: 3.0 µm silica
shell: 0.2 µm porous silica

pore size: 400 Å
application: proteins and polypeptides
range: MW 2 kDa – 500 kDa

 

  Dimension HALO®-5
Protein C4
HALO®
Protein ES-C18
  0.075 x 50 mm 8AM-94319-414 8AM-94319-402
  0.075 x 150 mm 8AM-94319-714 8AM-94319-702
  0.10 x 50 mm 8AM-94318-414 8AM-94318-402
  0.10 x 150 mm 8AM-94318-714 8AM-94318-702
  0.20 x 50 mm 8AM-94317-414 8AM-94317-402
  0.20 x 150 mm 8AM-94317-714 8AM-94317-702
  0.30 x 50 mm 8AM-94316-414 8AM-94316-402
  0.30 x 150 mm 8AM-94316-714 8AM-94316-702
  0.50 x 50 mm 8AM-94315-414 8AM-94315-402
  0.50 x 150 mm 8AM-94315-714 8AM-94315-702
  1.0 x 30 mm 8AM-93411-314 8AM-93411-302
  1.0 x 50 mm 8AM-93411-414 8AM-93411-402
  1.0 x 100 mm 8AM-93411-614 8AM-93411-602
  1.0 x 150 mm 8AM-93411-714 8AM-93411-702
  2.1 x 20 mm 8AM-93412-214 8AM-93412-202
  2.1 x 30 mm 8AM-93412-314 8AM-93412-302
  2.1 x 50 mm 8AM-93412-414 8AM-93412-402
  2.1 x 75 mm 8AM-93412-514 8AM-93412-502
  2.1 x 100 mm 8AM-93412-614 8AM-93412-602
  2.1 x 150 mm 8AM-93412-714 8AM-93412-702
  3.0 x 30 mm 8AM-93413-314 8AM-93413-302
  3.0 x 50 mm 8AM-93413-414 8AM-93413-402
  3.0 x 75 mm 8AM-93413-514 8AM-93413-502
  3.0 x 100 mm 8AM-93413-614 8AM-93413-602
  3.0 x 150 mm 8AM-93413-714 8AM-93413-702
  4.6 x 30 mm 8AM-93414-314 8AM-93414-302
  4.6 x 50 mm 8AM-93414-414 8AM-93414-402
  4.6 x 75 mm 8AM-93414-514 8AM-93414-502
  4.6 x 100 mm 8AM-93414-614 8AM-93414-602
  4.6 x 150 mm 8AM-93414-714 8AM-93414-702
             

HALO® Protein C4 & Protein ES-C18
guard columns

Guard column holder: 8AM-94900-001

  2.1 x 5 mm 8AM-93412-114 8AM-93412-102
  3.0 x 5 mm 8AM-93413-114 8AM-93413-102
  4.6 x 5 mm 8AM-93414-114 8AM-93414-102
       

HALO® BioClass
UHPLC & HPLC columns
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HALO® Columns
2.7 µm - 90Å
HALO®-5 Columns
5 µm - 90Å
HALO®-2 Columns
2 µm - 90Å
HALO® BioClass colums
for the separation of
peptides & proteins

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