HPLC columns & accessories

HALO® HPLC columns for UHPLC at "normal" pressure

 

The HALO® column allows the fast separation as in UHPLC but at "normal" pressure under 400 bar on standard HPLC instruments.

The fast separation is achieved by smaller size particles packed into the column which reduces the diffusion of solutes into and out of the stationary phase and therefore reduces peak broadening. This allows faster flow rates, fast separations and higher sample throughput. Unfortunately the smaller particles increase back pressure excessively and "fast" columns with 1.7 µm particles usually require expensive ultra-high pressure instrumentation.

 

The HALO® Column is based on the Fused-Core particle technology.

A porous shell is fused to a solid core particle. The shorter diffusion path of the HALO® particles reduces axial dispersion of solutes and minimizes peak broadening, which compared to a totally porous particle, allows faster flow rates.

Because of the increased separation power of the HALO® column, it can either be used for faster analysis or an improved separation

available phases:  

HALO® C18
C18 is used to separate analytes differing in hydrophobicity

HALO® C8
to separate analytes differing in hydrophobicity but interactions on C8 phases are weaker than on C18 phases

HALO® PFP
particularly well suited for the separation of halogenated compounds, nitro-aromatic compounds and polar bases

HALO® Phenyl-Hexyl
for the separation of aromatics and unsaturated analytes

HALO® RP Amid
especially for the separation of samples with acidic or alkaline components

HALO® ES-CN
provides strong dipole-dipole interaction and weak hydrophobic binding interaction with analytes. Typically used for reversed phase separations, but can also be used as a stationary phase for HILIC and normal-phase modes of separation.

HILIC & Penta-HILIC
particularly recommended for the separation of highly polar analytes that are poorly retained in reversed phase mode; also recommended for LC-MS applications because of the MS friendly mobile phase conditions typically used with Penta-HILIC

HALO® 2.7 µm Fused-Core column for HPLC and UHPLC separations

Silica phase; 2.7 µm - 90Å
provides the efficiency of sub-2µm particles at "normal" pressure under 400 bar

Also available as semi-preparative columen with ID 10 mm.

HALO® 5 µm Fused-Core column for HPLC separtions

Silica phase; 5 µm - 90Å
provides the efficiency of 3 µm particles with the pressure of 5 µm particles

Also available as semi-preparative columen with ID 10 mm.

HALO® 2 µm Fused-Core column for UHPLC separation

Silica phase; 2 µm- 90Å
more than 300,000 plates per meter
rugged
higher separation power than totally porous sub-2 µm columns

HALO® BioClass Fused-Core column for the separation of peptides and proteins

HALO® Protein C4 HALO® Protein ES-C18
for the fast separation of proteins and polypeptides up to Mw 500 kDa

HALO® Peptide ES-C18 HALO® Peptide ES-CN
for the fast separation of peptidesand polypeptides up to 10 kDa and 20 kDa

   

Optimize EXP™ hardware and accessories
EXP = Extreme Pressure Chromatography

EXP titanium/PEEK hybrid ferrule offering repeated zero-dead-volume connections, hand-tight to 800 bar or wrench tight to 1400 bar EXP holders and cartridges with ultra-low internal swept volume and hand-tight cartridge changeover for use to 1400 bar

● EXP™ titan hybrid ferrule
● EXP™ fittings & hex head EXP™ fittings
● EXP™ pre-column Filter
● EXP™ guard cartridge holder & cartridges
● EXP™ Trap columns

description & catalogue

   

Shodex columns

description & catalogue

Column storage box for HPLC columns

description

NP - amino phase
Asahipak

RP - polymer phase
Asahipak, RSpak

Ion chromatography
for strong or weak anions or anion or cation exchange
Shodex IEC Series

Sugar Analysis
Sugar can be separated by SEC, RP, NP, Ligand Exchange or Ion Exchange Chromatography
SUGAR S Series
SUGAR KS Series
Asahipak GS-220 HQ
Asahipak NHP2P Serie
s

Keep track of your the HPLC columns you have in stock with the column storage box, storing up to 30 HPLC columns.

bench-top model for 30 columns up to 300 mm length

● suitable for all end-fitting types

● solid steel construction with chrome plated handles

● stackable, thus easy to increase the number of columns in storage

● one drawer stores up to 10 columns x 12.5 cm length or a combination of columns, guard columns and analytical columns

   
HPLC Columns
& Accessories
Index
HALO® Columns
2.7 µm - 90Å
HALO®-5 Columns
5 µm - 90Å
HALO®-2 Columns
2 µm - 90Å
HALO® BioClass colums
for the separation of
peptides & proteins

HALO® applications
HALO® catalogues
Optimize EXP™
Hardware & Accessories
Shodex Columns
Description
& Catalogues
HPLC Column
Storage Box
ColStor
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