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HALO® Bioclass - large molecules
HALO® Bioclass columns are designed for fast and high resolution separations of numerous biomolecules.
Specifically designed for bioanalysis, with both tailored particle and pore size options for such analyses, the HALO® Bioclass columns are ideal for:
- Intact proteins, monoclonal antibodies (mAbs), biosimilars, and other large biomolecules such as pegylated proteins, antibody drug conjugates (ADCs), etc.
- Peptide mapping (analyses of enzyme digests) for characterisation and monitoring of synthetic protein drugs
- Analyses of therapeutic peptides and peptide biomarkers (protein surrogates)
- High resolution separations of complex mixtures of glycans released from N- and O-linked glycoproteins
HALO® Bioclass columns are available with particle sizes:
HALO® Bioclass Protein - 2.7 µm, 3.4 µm
HALO® Bioclass Peptide - 2.0 µm, 2.7 µm, 5.0 µm
HALO® Bioclass Glycan - 2.7 µm
Find more details to the individual column type in the tabs below.
HALO® BIOCLASS Protein
For the perfect large molecule solution, the HALO® BIOCLASS columns are available with 400 Å and 1000 Å.
Benefits of HALO® BIOCLASS Protein solutions include:
- provides narrower peaks and better recoveries for large biomolecules (vs. smaller pore sizes and non-core particles)
- allows HALO® BIOCLASS Protein columns to be used with both UHPLC and HPLC instrumentation for fast bioseparations at moderate back pressures
- C4 and sterically-protected ES-C18 phases
- excellent high temperature stability (up to 90 ̊C) for improved peak shape and recovery
- 2 μm inlet frit
- pressure limit, 600 bar/9000 psi
HALO® BIOCLASS Peptide
- extremely stable at high temperatures and low pH
- ideal for both ultrafast and ultrahigh resolution separations of peptides and polypeptides up to 20 kDa
- outperforms non-core 3 μm, 300 Angstrom columns in terms of peak width, peak capacity and peak height
- offers comparable peak capacity to sub-2 μm non-core columns at 40–50% back pressure (2.7 μm)
- ~ 20 % higher peak capacity than sub-2 μm non-core columns at comparable back pressure (2 μm)
- columns (Peptide 2.7 and 5 μm) can be used in series to increase peak capacity for UHPLC and HPLC analyses of complex tryptic digest samples
- HALO® Peptide ES-CN (2.7 and 5 μm) offers different selectivity and improved retention for polar peptides
- inlet frit provides extra protection from plugging:
2 µm inlet frit for 2.7 and 5 µm columns
1 µm inlet frit for 2 µm columns
HALO® BIOCLASS Glycan
- 90 Ångstrom pore size
- incorporates a highly polar ligand that contains 5 hydroxyl groups tethered to 2.7 μm Fused-Core silica particles via novel, proprietary linkage chemistry
- ideal for hydrophilic interaction liquid chromatography (HILIC) separations of oligosaccharides, and particularly, of released and labeled glycans from glycoproteins and proteoglycans
- 2 μm inlet frit
- Pressure limit, 600 bar/9000 psi
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HALO® Bioclass - large molecules
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Filter
Phase
Diphenyl
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
50 mm
Phase
Diphenyl
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
50 mm
Phase
ES-C18
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
100 mm
Phase
Diphenyl
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
100 mm
Phase
ES-C18
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
150 mm
Phase
Diphenyl
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
150 mm
Phase
ES-C18
Particle size
2.7 µm
Pore size
1000 Å
Inner Diameter
4.6 mm
Length
250 mm