The new HALO® PCS C18

In LC and LCMS separations, tailed peak shapes with basic compounds at high concentrations is a problem commonly observed when low ionic strength mobile phases, such as those using formic acid are employed. A way to improve the peak shape and sample load capacity is to use a stationary phase containing a positive charge.  The new HALO^® PCS line of columns are positively charged surface chemistries which maintain peak symmetry at higher loading capacities and provide alternate selectivities from other  C18 and Phenyl-Hexyl bonded phases.

Built upon proven Fused-Core^® technology  and available in both a 90 Å and 160 Å pore size for small molecule and peptide analysis, the columns are optimized to deliver performance for reproducible, high efficiency LC and LCMS separations.

The advantages of the HALO^® PCS C18

• high lot-to-lot reproducibility
• excellent stability

Your profits when using the HALO^® PCS C18

• Increased sample load with basic compounds in comparison to fully porous C18 columns
• Higher resolution, improved tailing factor and more plates in comparison to competitors’ SPP C18+ columns
• Improved peak shape and resolution for peptide in comparison to other C18 phases

Ideal applications:

• Reversed phase separations of basic analytes prone to peak tailing
• Separation of beta blockers such as Atenolol, Pindolol, Nadolol, Metoprolol, etc.
• Fast separation of basic antidepressants such as Nortriptyline, Amitriptyline, Trimipramine, etc,
• Higher resolution and higher sample load for peptides
• Peptide mapping

Further information and application examples

• White Paper: HALO® PCS: New Column Chemistry Options for Basic Compounds
• What to Expect from HALO® PCS C18
• Improved Bioseparations with HALO® PCS C18 PEPTIDE
• HALO 90 Å PCS C18 Compared to Competitor SPP Charged Surface Phase

• Separation of Antidepressants Using HALO 90 Å PCS C18
• Beta Blockers Separation on HALO® PCS C18
• HALO 160 Å PCS C18 Rapid Peptide Separation
• HALO 160 Å PCS C18 vs. C18 Peptide Panel
• HALO 160 Å PCS C18 Loading Studies

Order information

Order information